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biogas HANDBOOK Accumulated biogas yield (m3/kg) Specific gas production rate (m3/m3*d) Average hydraulic retention time (HRT), in days Figure 3.6 Biogas production after addition of substrate –batch test (LfU 2007) 3.2.1 Hydrolysis Hydrolysis is theoretically the first step of AD, during which the complex organic matter (polymers) is decomposed into smaller units (mono- and oligomers). During hydrolysis, polymers like carbohydrates, lipids, nucleic acids and proteins are converted into glucose, glycerol, purines and pyridines. Hydrolytic microorganisms excrete hydrolytic enzymes, converting biopolymers into simpler and soluble compounds as it is shown below: Lipids →fattyacids,glycerol cellulase, cellobiase, xylanase, amylase lipase Polysaccharide → monosaccharide protease Proteins → amino acids A variety of microorganisms is involved in hydrolysis, which is carried out by exoenzymes, produced by those microorganisms which decompose the undissolved particulate material. The products resulted from hydrolysis are further decomposed by the microorganisms involved and used for their own metabolic processes. 3.2.2 Acidogenesis During acidogenesis, the products of hydrolysis are converted by acidogenic (fermentative) bacteria into methanogenic substrates. Simple sugars, amino acids and fatty acids are degraded into acetate, carbon dioxide and hydrogen (70%) as well as into volatile fatty acids (VFA) and alcohols (30%). 3.2.3 Acetogenesis Products from acidogenesis, which can not be directly converted to methane by methanogenic bacteria, are converted into methanogenic substrates during acetogenesis. VFA 22 Gas production rate or biogas yieldPDF Image | biogas HANDBOOK
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